Nematode promotes reprogramming in tomato plants

Study revealed significant changes in tomato genetic patterns (Solanum lycopersicum) when infected by root-knot nematode Meloidogyne incognita. The researchers identified changes in gene expression and alternative RNA splicing in roots, both locally in galls and in adjacent cells.

The work showed that the nematode manipulates genetic processes in the early and late stages of infection. At 4 days post infection (dpi), 3895 genes were differentially expressed in galls and 6944 in adjacent cells. At 11 dpi, the numbers were 3582 and 1063 genes, respectively.

Furthermore, more than 9000 alternative splicing events were identified that modify gene functioning, with a direct impact on gall formation and nematode egg production.

The researchers found that the nematode suppresses tomato defense responses, especially early in the infection, to create an environment favorable to parasitism.

It was also observed that nematodes stimulate disordered cell division in the galls, resulting in metabolically active giant cells, essential for the parasite's nutrition.

The research used RNA-seq analyses in infected and control roots. Genetic manipulation of model plants confirmed the role of alternative splicing in plant-nematode interactions.

Genes that underwent alternative modification had a direct impact on gall formation and nematode reproduction.

More information can be found at doi.org/10.1093/hr/uhae206

Information on management of Meloidogyne incognita in tomato plants can be obtained by clicking on "integrated nematode management in tomato"