Fungicides increase wheat seed protection

Cooperative trials in six Brazilian wheat-growing regions indicate formulations with superior control

03.07.2025 | 15:40 (UTC -3)
Cultivar Magazine
Photo: Jack Dykinga/USDA
Photo: Jack Dykinga/USDA

The analysis of fungicides in the treatment of wheat seeds, conducted in six regions of Brazil in the 2024/25 harvest, confirmed the high efficacy of specific formulations against pathogens Fusarium graminearum e Bipolaris sorokiniana. The trials were part of the Cooperative Trials Network and used seeds from the 2023 harvest, marked by a high incidence of diseases caused by excess moisture.

Mixtures with benzimidazoles demonstrated control of up to 97,9% over F. graminearum. The combination of fluxapyroxad with mefentrifluconazole exceeded 96% control of B. sorokiniana. Products such as pyraclostrobin + thiophanate-methyl + fipronil and thiophanate-methyl + fluazinam maintained control of over 85% for both pathogens.

Seeds compromised by the previous harvest

The origin of the seeds significantly influenced the rates of natural infection. Samples from Cafelândia (PR) showed 64,8% contamination by F. graminearum. In Palmeira (PR), the highest incidence of B. sorokiniana reached 6%.

This health condition reflected the environment of the 2023 harvest, when heavy rains favored diseases such as head blight and leaf spots. Exposure to these pathogens compromised seed quality, increasing the risk of infection in the new crop.

Objective and methodology of the tests

The researchers evaluated nine fungicidal treatments against F. graminearum and eight against B. sorokiniana, in addition to positive and negative controls. The seeds were inoculated to simulate artificial infections of up to 20%. The evaluation followed the “blotter test” protocol, with seed incubation and microscopic analysis of the presence of fungal structures.

Each treatment was applied according to the manufacturer's recommendations, with 600 mL of solution per 100 kg of seeds. The statistical analysis used mixed linear models and the Tukey test (5%) to compare the effectiveness of the products.

Technical performance

in control of F. graminearum, the average natural infection rate was 7,8%. For B. sorokiniana, the average natural infection was 1,0%.

Among the treatments with good performance against both pathogens, two formulations stood out:

  • pyraclostrobin + thiophanate-methyl + fipronil
  • thiophanate-methyl + fluazinam

Both showed efficacy of over 85% in controlling the two diseases. The combination of ingredients allowed for a broader spectrum of action, with a significant reduction in the seed infection rate.

Treatments used in trials conducted in the Cooperative Wheat Trial Network to control the fungus via seed treatment of wheat ( )
Treatments used in trials conducted in the Wheat Cooperative Trial Network for fungus control Fusarium graminearum via wheat seed treatment (Triticum aestivum)
Means and standard error for incidence of in wheat ( ) seeds and estimated control efficiency for different fungicide treatments. (1) T1 = natural occurrence of fungi in seeds without inoculation; T2 = positive control; T3 = standard chemical control (pyraclostrobin + thiophanate-methyl + fipronil); T4 = fluxapyroxide and mefentrifluconazole; T5 = thiophanate-methyl + fluazinam; T6 = chlorothalonil + thiophanate-methyl; T7 = carboxin + thiram; T8 = thiophanate-methyl + fluazinam; T9 = metalaxyl-M + thiabendazole + fludioxonil. (2) Means that do not share letters in common differ significantly from each other, according to the Tukey test performed at the 5% significance level. Prior to analysis, incidence data were square root transformed. (3) Percentage of control relative to treatment T2 (inoculated with the pathogen). (4) Treatment T1 did not perform statistical analysis as it was not subject to comparison with the other treatments.
Means and standard error for incidence of Fusarium graminearum in wheat seeds (Triticum aestivum) and estimated control efficiency for different fungicide treatments. (1) T1 = natural occurrence of fungi in seeds without inoculation; T2 = positive control; T3 = standard chemical control (pyraclostrobin + thiophanate-methyl + fipronil); T4 = fluxapyroxide and mefentrifluconazole; T5 = thiophanate-methyl + fluazinam; T6 = chlorothalonil + thiophanate-methyl; T7 = carboxin + thiram; T8 = thiophanate-methyl + fluazinam; T9 = metalaxyl-M + thiabendazole + fludioxonil. (2) Means that do not share letters in common differ significantly from each other, according to the Tukey test performed at a significance level of 5%. Prior to analysis, incidence data were transformed by square root. (3) Percentage of control relative to treatment T2 (inoculated with the pathogen). (4) Treatment T1 did not perform statistical analysis as it was not subject to comparison with the other treatments.
Treatments used in trials conducted in the Cooperative Wheat Trials Network for controlling the fungus via seed treatment of wheat ( )
Treatments used in trials conducted in the Wheat Cooperative Trial Network for fungus control Bipolaris sorokiniana via wheat seed treatment (Triticum aestivum)
Means and standard error for incidence of in wheat ( ) seeds and estimated control efficiency for different fungicide treatments. (1) T1 = natural occurrence of fungi in seeds without inoculation; T2 = positive control; T3 = standard chemical control (pyraclostrobin + thiophanate-methyl + fipronil); T4 = fluxapyroxide and mefentrifluconazole; T5 = thiophanate-methyl + fluazinam; T6 = chlorothalonil + thiophanate-methyl; T7 = carboxin + thiram; T8 = thiophanate-methyl + fluazinam; T9 = metalaxyl-M + thiabendazole + fludioxonil. (2) Means that do not share letters in common differ significantly from each other, according to the Tukey test performed at the 5% significance level. Prior to analysis, incidence data were square root transformed. (3) Percentage of control relative to treatment T2 (inoculated with the pathogen). (4) Treatment T1 did not perform statistical analysis as it was not subject to comparison with the other treatments.
Means and standard error for incidence of Bipolaris sorokiniana in wheat seeds (Triticum aestivum) and estimated control efficiency for different fungicide treatments. (1) T1 = natural occurrence of fungi in seeds without inoculation; T2 = positive control; T3 = standard chemical control (pyraclostrobin + thiophanate-methyl + fipronil); T4 = fluxapyroxide and mefentrifluconazole; T5 = thiophanate-methyl + fluazinam; T6 = chlorothalonil + thiophanate-methyl; T7 = carboxin + thiram; T8 = thiophanate-methyl + fluazinam; T9 = metalaxyl-M + thiabendazole + fludioxonil. (2) Means that do not share letters in common differ significantly from each other, according to the Tukey test performed at a significance level of 5%. Prior to analysis, incidence data were transformed by square root. (3) Percentage of control relative to treatment T2 (inoculated with the pathogen). (4) Treatment T1 did not perform statistical analysis as it was not subject to comparison with the other treatments.

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